UC San Diego Lipidomics Core Services

1. Fatty Acids: analyzed by GC-MS and fully quantitated by comparison with authentic standards. Currently, the fatty acid panel consists of 33 fatty acids, ranging in chain length from 12 to 26 carbons, and includes all saturated fatty acids, mono- and poly-unsaturated fatty acids and their positional isomers
   1. Free (non-esterified) Fatty Acid Panel
   2. Total (sum total of esterified and non-esterified) Fatty Acid Panel
2. Eicosanoids: Eicosanoids and N-acylethanolamines are analyzed by UPLC-MS/MS and are fully quantitated by comparison with authentic standards. The Comprehensive Eicosanoid Panel is a global eicosanoid profile, presently consisting of over 150 eicosanoids. The 10-Eicosanoid Panel is a client-defined set of 10 eicosanoids (contact us for a list of eicosanoids to choose from).
   1. Comprehensive Eicosanoid Panel
   2. 10-Eicosanoid Panel
3. Acylcarnitines: play important roles in fatty acid metabolism and function as vehicles to transport acyl groups from the cytosol into the mitochondria for β-oxidation. Acylcarnitines are analyzed by untargeted UPLC-MS/MS approaches and are identified and quantified using authentic standards.
   1. Acylcarnitine Panel

1. Phospholipid Panel: analyzes all individual species contained within each phospholipid class and employs sophisticated acquisition protocols that will provide the exact fatty acid composition of each lipid molecular species. For routine analysis, we provide absolute amounts of each lipid molecular species based on internal standards that added at exactly known amounts. The semi-quantitative results allow for direct comparison between samples or treatments. On special request, we can also quantitate the isobaric species based on existing standards.
2. Phospholipid Class Panel: consists of a quantitative phospholipid class scan that includes phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidic acid, phosphatidylglycerol and all lyso derivatives of these. We can quantify all of these classes or limit to only the ones that you select.

1. Glycerolipid Panel: provides data on all individual triacylglyceride and diacylglyceride molecular species that differ in their fatty acid composition. Due to the limited availability of quantitative standards, we recommend glycerolipids profiling scans at the semi-quantitative level, which allows direct comparison between samples or treatments. On special request, we can also quantitate all molecular species based on existing standards. 

1. Sphingolipid Panel: consists of both the Sphingomyelin Panel and the Ceramide Panel and provides quantitative data on all lipid molecular species based on internal standards added at exactly known concentrations.
2. Sphingomyelin Panel: provides quantitative data on all lipid molecular species based on internal standards added at exactly known concentrations.
3. Ceramide Panel: provides quantitative data on all lipid molecular species based on internal standards added at exactly known concentrations.
4. Sphingoid Bases Panel: includes sphingosine, sphinganine, sphingosine-1-phosphate, sphinganine-1-phosphate, 1-deoxysphinganine, 1-deoxysphingosine and 3-ketosphinganine. These measurements are performed fully quantitatively.

1. Cholesterol Ester Panel: for the analysis of cholesterol esters, we use a MS protocol that yields profiling data on all individual cholesterol ester molecular species based on the associated fatty acids and we provide fully quantitative data. 

1. Free (non-esterified) Sterol Panel
2. Total (sum total of esterified and non-esterified) Sterol Panel

1. Complex Lipid Panel: includes the Phospholipid Panel, the Glycerolipid Panel, the Sphingolipid Panel, the Cholesterol Ester Panel and the Acylcarnitine Panel. 

1. Extraction
2. Homogenization (of solid tissue)
3. Protein Analysis

Dr. Oswald Quehenberger is available to provide additional consultation for projects, including more detailed lipid metabolism information, sample preparation instructions, experimental design and setup, and data interpretation. Consultation is billed at an hourly rate.

Dr. Oswald Quehenberger
Email: oquehenberger@health.ucsd.edu